人类心室心肌细胞 (hvCM)
分化自多能干细胞的高纯度丶成熟的人类心室心肌细胞
高纯度高品质

高纯度 hvCM,品质控制严格到位。

定义的功能

表征良好的电生理现象、转录组以及蛋白质组。

细胞应用

手动膜片钳和药物筛查应用随时可用。

人类心室心肌细胞 (hvCM)分化自多能干细胞,丶成熟且纯度高。
我们顾客可以选择使用Novoheart提供的标准人类多能干细胞组或顾客可自行提供他們的人类多能干细胞组样本給我們製作反映不同基因背景的人类心肌细胞 (如需特別订做細胞请直接联络我們)。

        - 細胞能由冷冻保存迅速復原並保持細胞还原度超过八成
        - 表征优秀的电生理现象、转录组以及蛋白质组

我们有两种人类心肌细胞可供选购:

  • (生产批号:1-01) 未纯化人类心肌细胞 (>70% 纯度)
  • (生产批号:1-02) 已纯化人类心肌细胞
  • 心脏致毒成分筛查。
  • 药物研发, 药物筛查及疗效评估。
  • 再生研究: 在动物上实验的器官移植, 经手动或自膜片钳的单细胞分析, 电压或Ca2+敏感荧光团的光学成像。

Keung, W., Ren, L., Sen Li, Wong, A. O., Chopra, A., Kong, C. W., Tomaselli G. F., Chen, C. S., Li, R. A. Non-cell autonomous cues for enhanced functionality of human embryonic stem cell-derived cardiomyocytes via maturation of sarcolemmal and mitochondrial K(ATP) channels. Sci Rep. 6, 34154 (2016).


Poon, E., Keung, W., Liang, Y., Ramalingam, R., Yan, B., Zhang, S., Chopra, A., Moore, J., Herren, A., Lieu, D. K., Wong, H. S., Weng, Z., Wong, O. T., Lam, Y. W., Tomaselli, G. F., Chen, C., Boheler, K. R. & Li, R. A. Proteomic Analysis of Human Pluripotent Stem Cell-Derived, Fetal, and Adult Ventricular Cardiomyocytes Reveals Pathways Crucial for Cardiac Metabolism and Maturation. Circ Cardiovasc Genet 8, 427–436 (2015).


Zhang, S., Poon, E., Xie, D., Boheler, K. R., Li, R. A., Wong, H. S. Consensus comparative analysis of human embryonic stem cell-derived cardiomyocytes. PLoS One. 10, e0125442 (2015).


Chen, G., Li, S., Karakikes, I., Ren, L., Chow, M. Z., Chopra, A., Keung, W., Yan, B., Chan, C. W., Costa, K. D., Kong, C. W., Hajjar, R. J., Chen, C. S., Li, R. A. Phospholamban as a crucial determinant of the inotropic response of human pluripotent stem cell-derived ventricular cardiomyocytes and engineered 3-dimensional tissue constructs. Circ Arrthyhm Electrophysiol. 8, 193-201 (2015).


Li, R. A. Cardiovascular regeneration. Stem Cell Res Ther. 5, 141 (2014).


Weng, Z., Kong, C.-W., Ren, L., Karakikes, I., Geng, L., He, J., Chow, M. Z. Y., Mok, C. F., Chan, H. Y. S., Webb, S. E., Keung, W., Chow, H., Miller, A. L., Leung, A. Y. H., Hajjar, R. J., Li, R. A. & Chan, C. W. A Simple, Cost-Effective but Highly Efficient System for Deriving Ventricular Cardiomyocytes from Human Pluripotent Stem Cells. Stem Cells Dev. 23, 1704–1716 (2014).


Keung, W., Boheler, K. R., Li, R. A., Developmental cues for the maturation of metabolic, electrophysiological and calcium handling properties of human pluripotent stem cell-derived cardiomyocytes. Stem Cell Res Ther. 5, 17, (2014).


Karakikes, I., Senyel, G. D., Hansen, J., Kong, C.-W., Azeloglu, E. U., Stillitano, F., Lieu, D. K., Wang, J., Ren, L., Hulot, J.-S., Iyengar, R., Li, R. A. & Hajjar, R. j. Small Molecule-Mediated Directed Differentiation of Human Embryonic Stem Cells Toward Ventricular Cardiomyocytes. Stem Cells Transl. Med. 3, 18–31 (2014).


Li, S., Cheng, H., Tomaselli, G. F., Li, R. A. Mechanistic basis of excitation-contraction coupling in human pluripotent stem cell-derived ventricular cardiomyocytes revealed by Ca2+ spark characteristics: direct evidence of functional Ca2+-induced Ca2+ release. Heart Rhythm. 11, 133-140 (2014).


Li, S., Chen, G., Li, R. A. Calcium signalling of human pluripotent stem cell-derived cardiomyocytes. J Physiol. 591, 5279-5290 (2013).


Poon, E., Yan, B., Zhang, S., Rushing, S., Keung, W., Ren, L., Lieu, D. K., Geng, L., Kong, C. W., Wang, J., Wong H. S., Boheler, K. R., Li, R. A. Transcriptome-guided functional analyses reveal novel biological properties and regulatory hierarchy of human embryonic stem cell-derived ventricular cardiomyocytes crucial for maturation. PLoS One. 8, e77784 (2013).


Chow, M. Z., Geng, L., Kong, C. W., Keung, W., Fung, J. C., Boheler, K. R., Li, R. A. Epigenetic regulation of the electrophysiological phenotype of human embryonic stem cell-derived ventricular cardiomyocytes: insights for driven maturation and hypertrophic growth. Stem Cells Dev. 22, 2678-2690 (2013).


Chow, M., Boheler, K. R., Li, R. A. Human pluripotent stem cell-derived cardiomyocytes for heart regeneration, drug discovery and disease modelling: from the genetic, epigenetic, and tissue modeling perspective. Stem Cell Res Ther. 4, 97 (2013).


Lieu, D. K., Fu, J. D., Chiamvimonvat, N., Tung, K. C., McNerney, G. P., Huser, T., Keller, G., Kong, C. W., Li, R. A. Mechanism-based facilitated maturation of human pluripotent stem cell-derived cardiomyocytes. Circ Arrhythm Electrophysiol. 6, 191-201 (2013).


Fu, J. D., Rushing, S. N., Lieu, D. K., Chan, C. W., Kong, C. W., Geng, L., Wilson, K. D., Chiamvimonvat, N., Boheler, K. R., Wu, J. C., Keller, G., Hajjar, R. J., Li, R. A. Distinct roles of microRNA-1 and -499 in ventricular specification and functional maturation of human embryonic stem cell-derived cardiomyocytes. PLoS One. 6, e27417 (2011).


Wilson, K. D., Hu, S., Venkatasubrahmanyam, S., Fu, J. D., Sun, N., Abilez, O. J., Baugh, J. J., Jia, F., Ghosh, Z., Li, R. A., Butte, A. J., Wu, J. C. Dynamic microRNA expression programs during cardiac differentiation of human embryonic stem cells: role for miR-499. Circ Cardiovasc Genet. 3, 426-435 (2010).


Fu, J. D., Jiang, P., Rushing, S., Liu, J., Chiamvimonvat, N., Li, R. A. Na+/Ca2+ exchanger is a determinant of excitation-contraction coupling in human embryonic stem cell-derived ventricular cardiomyocytes. Stem Cells Dev. 19, 773-782 (2010).


Liu, J., Lieu, D. K., Siu, C. W., Fu, J. D., Tse, H. F., Li, R. A. Facilitated maturation of  Ca2+ handling properties of human embryonic stem cell-derived cardiomyocytes by calsequestrin expression. Am J Physiol Cell Physiol. 297, C152-159 (2009).


Lieu, D. K., Liu, J., Siu, C. W., McNerney, G. P., Tse, H. F., Abu-Khalil, A., Huser, T., Li, R. A. Absence of transverse tubules contributes to non-uniform Ca(2+) wavefronts in mouse and human embryonic stem cell-derived cardiomyocytes. Stem Cells Dev. 18, 1493-1500 (2009).


Chan, J. W., Lieu, D. K., Huser, T., Li, R. A. Label-free separation of human embryonic stem cells and their cardiac derivatives using Raman spectroscopy. Anal Cham. 81, 1324-1331 (2009).

生产批号 产品名称 数量
1-0101 未纯化人类心肌细胞 (>70% 纯度) 每瓶有超过500万活存細胞 查询报价
1-0102 未纯化人类心肌细胞 (>70% 纯度) 每瓶有超过1000万活存細胞 查询报价
1-0201 已纯化人类心肌细胞 每瓶有超过500万活存細胞 查询报价
1-0202 已纯化人类心肌细胞 每瓶有超过1000万活存細胞 查询报价

How are the cardiomyocytes stored and shipped?
Cardiomyocytes are harvested on Day 15 post-differentiation, isolated and suspended in DMSO, and cryopreserved at -80oC in 1.5mL cryovials containing 106 cells each. The cells are shipped frozen.

How are the cardiomyocytes differentiated from human pluripotent stem cells?
The cardiomyocytes are differentiated using our patent-pending small molecule-based differentiation method. Please see our publications Stem Cells Transl Med 3, 18-31 (2014) and Stem Cells Dev 23, 1704-1716 (2014) for details.

What types of characterization have been performed on the cardiomyocytes?
Manual patch-clamp recordings of action potentials and ionic currents, optical recordings of action potentials and Ca2+ transients, immunofluorescent imaging, transcriptomic analysis, and proteomic analysis have been performed to characterize the cells.



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